The GenapSys Sequencing Prep System automates clonal amplification with only minutes of hands-on time.

Streamlined 4-step workflow using the GenapSys Platform.

The system utilizes the first ever purely electrical sequencing chip, the key component in our fully integrated proprietary platform.

Our proprietary NGS technology with proven Sequencing-by-Synthesis chemistry employs electrical-based detection of single nucleotide incorporations. With CMOS-based detectors (Complementary Metal Oxide Semiconductor), simple fluidics, and no moving parts, the GenapSys Sequencer is compact, robust, easy to use, and inexpensive.

GenapSys™ has developed a novel electrical-based platform capable of accurately detecting single base incorporations. On the surface of the CMOS sequencing chip (Complementary Metal Oxide Semiconductor), there are millions of sensors, each designed to capture one clonally amplified bead. Every sensor consists of electrodes in close proximity to each other, both of which contact the bead captured by that sensor.

Since nucleotide incorporation into a growing DNA strand increases the amount of charged molecules around the bead, it changes the impedance measured by the sensor. The magnitude of the impedance change is correlated to the total change in the charge of DNA molecules and their surrounding layers around the bead, which is correlated to the number of incorporated nucleotides.

The plot contains a representative example of acquired data showing the distribution of measured differential signal across multiple nucleotide flows for a single template sequence. Flows that are expected to have nucleotide incorporations are highlighted in red. Non-incorporating flows are shown in blue and do not deviate significantly from the baseline. The inferred sequence, including the number of nucleotides in each incorporation are shown below.

While almost all other NGS technologies are based on transient measurements, our detection modality is based on steady state measurements. Due to its steady state nature, the signal stays constant and we can take multiple measurements to increase our precision and signal-to-noise (SNR) which significantly improves our base calling accuracy. Also, the design of the chip allows for very low cross-talk between the sensors which enables high density packing of the sensors.