WOLF Cell Sorter Consumables

Discover the WOLF microfluidic cartridges for all your cell sorting applications.

Technology

Better than a fluidic cart, sterile microfluidic cartridges ensure low-pressure cell sorting applications for sensitive cells. With disposable cartridges, there will be no more sample-to-sample contamination

Unique to NanoCellect are our disposable cartridges that allow for bulk sorting or single-cell sorting. The sorting cartridges use a piezoacoustic actuator that gently directs cells into collection channels; an embedded cell sorting verification system gives instant feedback of sorting accuracy. And because the cartridges are disposable, there is no chance of sample-to-sample contamination or biohazardous aerosols.

NanoCellect offers two types of cartridges. First for bulk sorting, the cartridge has 3 output channels that allow to sort 2 selected cell populations in the A and C channels while the remainder of the cells are collected in the B channels. The single-cell sorting cartridge is designed to work with the N1 Single-Cell Dispenser accessory to deposit 1 to 100 cells per well of a 96- or 384-well plate.

Accessories and consumables

Highlights of NanoCellect’s Microfluidic Cartridges

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Sterile
Cartridges are individually-wrapped in sterile packing to avoid cross contamination.
Disposable
Anything that the sample or sheath fluid touches is disposable.
Gentle Sorting
A gentle sorting mechanism results in improved viability of cells and higher outgrowth.
No aerosols
No aerosols are produced during sorting.
Easy clean-up
Clean-up in 1 minute: once finished for the day simply discard the cartridge.

N1 Single-Cell Dispenser Accessory

A module for the WOLF Cell Sorter to sort and dispense into 96-well or 384-well plates.

The N1 Single-Cell Dispenser revolutionizes applications that demand precise isolation, sorting, and dispensing of individual cells. Whether it’s B-cell identification for antibody discovery or monoclonal CHO cell line development, the N1 Single-Cell Dispenser simplifies these tasks significantly. Researchers can now streamline single-cell genomics assays by directly plating single cells into PCR plates, unlocking new possibilities for high-throughput analyses. Achieve unparalleled accuracy and efficiency is single cell dispensing with the N1 Single-Cell Dispenser and transform the way you approach critical applications that rely on single-cell isolation.

High Viability

The N1 single-cell dispenser is ideal for a multitude of applications including single cell cloning and works seamlessly with the microfluidic-based WOLF cell sorter for easy single cell isolation, single cell analysis, and cell line development. The gentle low-pressure sorting mechanism and single cell manipulation preserve cell viability through the sorting process and provide higher outgrowth of healthy clones. Reduced stress via gentle microfluidic cell sorting (low throughput) process also avoids potential adverse gene expression or karyotype changes experienced with a traditional cell sorter.

Sterile Microfluidics

The WOLF uses a small fraction of the sheath fluid used by traditional sorters, allowing users to use growth media as sheath. Using growth media as sheath greatly improves cell viability for cell isolation, FACS (fluorescence activated cell sorting) and overall flow cytometry analysis.

Single Cell Efficiency
The WOLF provides 5 parameters for analyzing and sorting cells. When combined with the N1 Single-Cell Dispenser accessory, it allows higher rates in single cell detection in wells compared to cell printers or limited dilution methods. Deposition above 90-95% are routinely observed.
Easy-to-use
There is no need to be a flow cytometry expert in order to use the WOLF. The software interface is intuitive, user-friendly and simple. There is no fluidics cart, and the optics are fixed – resulting in less complexity, less clean-up, and more time for experiments.
Small Footprint
Compared to a traditional flow cytometry lab setup, the robust, portable, sterile, and safe benchtop system can be used in any lab without the need for dedicated hoods, venting, or personnel. Light enough to be moved and shared. Small enough to have both the WOLF and the N1 single-cell dispenser placed in a biosafety cabinet.
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